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KMID : 1041220130510010020
Journal of Sericultural and Entomological Science
2013 Volume.51 No. 1 p.20 ~ p.29
Quantitative Analysis of 1-deoxynojirimycin in Mulberry Leaves
Kim Hyun-Bok

Seo Sang-Deok
Koo Hui-Yeon
Seok Young-Seek
Kim Sun-Lim
Sung Gyoo-Byung
Abstract
Countries of the world are competing for the collection and utilization of genetic resources, which is a resource of value-added industrialization. We are building database of silkworm and mulberry resources with morphological, genetic characteristics data etc. Moreover, recent bioactive compound has been receiving increasing attention. So we analyzed 1-deoxynojirimycin(1-DNJ) content as a this item in addition to the basic information using 363 strains(varieties) of mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for 1-DNJ test. As a result, 1-DNJ mean content of 363 mulberry strains was 0.176 ¡¾ 0.077%, and the coefficient of variation (CV) was 43.5%. The variation between the strains was greatly severe. Among the tested strains, 'C1D89/29' was showed the highest content of 0.47%, whereas 'Pumbo 24' and 'Turkey E' were showed the lowest content of 0.05% respectively. The content of 1-DNJ of 16 mulberry varieties for silkworm rearing were compared. Average concentration was 0.17 ¡¾ 0.04%, and the coefficient of variation (CV) was 22.8%. Variation among cultivars was not significant. Finally we selected 1-DNJ high-containing 11 strains. They are as follows. 'C1D89/29', 'Hiihak', 'Jeokasibmunja', 'Gweonchil', 'Botongsibmunja', 'Jeokchuk', 'bulguksang', 'Geunsookgojo', 'Busanggeum', 'Guksang 20' and 'Taekwang' which are more than twice of the overall average content.
KEYWORD
Mulberry leaf, 1-Deoxynojirimycin(1-DNJ), Genetic resource
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